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KMID : 0361019700130040035
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Korean Journal of Otolaryngology - Head and Neck Surgery
1970 Volume.13 No. 4 p.35 ~ p.38
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Electron Microscopic Studies upon Normal Spiral Ganglion Cells of Guinea pigs
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Lim Jang-Sun
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Abstract
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Materials for these studies were taken from 6 healthy guinea pigs, 300 -350 gm in weight, which had revealed normal preyer s reflex. The modiolus and spiral lamina were exposed by careful removal of the bony capsule. The modiolus including cochlear nerve was brought immediately´ into 2% osmium tetraoxide adjusted at pH 7.4 with acetate veronal buffer. After fixation, they were dehydrated through a series of ethyl alcohol graded from :60%-100% for 20 minutes respectively.
After these procedures, bony portion of the modiolus and spiral lamina were trimming. Ultra-thin sections were cut from´Epon blocks by glass knives on :MT -2 Ultra-microtome, 400-600A think section and stained with saturated uracyl acetate solution and lead citrate solution for about 30 minutes and 15 minutes respectively. Electron-microscopic pictures of spiral ganglion cells were observed with Hitachi-He-11E-1 type electron microscope.
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